研究業績 - Publications


研究論文 Research Articles


CAMSAP2 is required for bridging fiber assembly to ensure mitotic spindle assembly and chromosome segregation in human epithelial Caco-2 cells
Naoko Nishizawa, Riku Arai, Koki Hiranuma, Mika Toya, *Masamitsu Sato
PLoS One. (2025) 20: article number: e0308150; DOI:10.1371/journal.pone.0308150


Chromosome arm length, and a species-specific determinant, define chromosome arm width
*Yasutaka Kakui, Christopher Barrington, Yoshiharu Kusano, Rahul Thadani, Todd Fallesen, Toru Hirota, *Frank Uhlmann
Cell Reports (2022) 41: article number: 111753; DOI:10.1016/j.celrep.2022.111753


Fission yeast Dis1 is an unconventional TOG/XMAP215 that induces microtubule catastrophe to drive chromosome pulling
Yuichi Murase, Masahiko Yamagishi, Naoyuki Okada, Mika Toya, Junichiro Yajima, Takahiro Hamada, *Masamitsu Sato
Communications Biology (2022) 5: article number: 1298; DOI:10.1038/s42003-022-04271-2


Structure-based screening for functional non-coding RNAs in fission yeast identifies a factor repressing untimely initiation of sexual differentiation
Yu Ono, Kenta Katayama, Tomoki Onuma, Kento Kubo, Hayato Tsuyuzaki, *Michiaki Hamada, *Masamitsu Sato
Nucleic Acids Research (2022) DOI:https://doi.org/10.1093/nar/gkac825


The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis
Hayato Hirai, Yuki Shogaki, *Masamitsu Sato
Communications Biology (2022) 5:article number: 818. 15 Aug; DOI:10.1038/s42003-022-03786-y


Tracheal motile cilia in mice require CAMSAP3 for formation of central microtubule pair and coordinated beating
Hiroko Saito, Fumiko Matsukawa-Usami, Toshihiko Fujimori, Toshiya Kimura, Takahiro Ide, Takaki Yamamoto, Tatsuo Shibata, Kenta Onoue, Satoko Okayama, Shigenobu Yonemura, Kazuyo Misaki, Yurina Soba, Yasutaka Kakui, Masamitsu Sato, Mika Toya, and Masatoshi Takeichi
Molecular Biology of the Cell (2021) 32(20):ar12 DOI:10.1091/mbc.E21-06-0303


Dual impact of a benzimidazole resistant β-tubulin on microtubule behavior in fission yeast
Mamika Minagawa, Minamo Shirato, Mika Toya, *Masamitsu Sato
Cells (2021) 10(5):1042. DOI: 10.3390/cells10051042


Simplification of nutritional conditions in transformation procedures for genome editing with the CRISPR/Cas9 system for fission yeast
Seibun Li, Mika Toya, *Masamitsu Sato
Gene (2021) 145595 DOI:10.1016/j.gene.2021.145595


Cyst formation in proximal renal tubules caused by dysfunction of the microtubule minus-end regulator CAMSAP3
Yuto Mitsuhata, Takaya Abe, Kazuyo Misaki, Yuna Nakajima, Keita Kiriya, Miwa Kawasaki, Hiroshi Kiyonari, *Masatoshi Takeici, *Mika Toya, Masamitsu Sato
Scientific Reports (2021) 11:5857 DOI:10.1038/s41598-021-85416-x


Comparison of loop extrusion and diffusion capture as mitotic chromosome formation pathways in fission yeast
Tereza Gerguri, Xiao Fu, Yasutaka Kakui, Bhavin S Khatri, Christopher Barrington, Paul A Bates, Frank Uhlmann
Nucleic Acids Research (2021) 49(3):1294-1312 DOI:10.1093/nar/gkaa1270


Fission yeast condensin contributes to interphase chromatin organization and prevents transcription-coupled DNA damage
Yasutaka Kakui, Christopher Barrington, David J. Barry, Tereza Gerguri, Xiao Fu, Paul A. Bates, Bhavin S. Khatri and Frank Uhlmann
Genome Biology (2020) 21(1):272 DOI:10.1186/s13059-020-02183-0


Time-lapse single-cell transcriptomics reveals modulation of histone H3 for dormancy breaking in fission yeast
Hayato Tsuyuzaki, Masahito Hosokawa, Koji Arikawa, Takuya Yoda, Naoyuki Okada, Haruko Takeyama and Masamitsu Sato
Nature Communications (2020) 11:1265 DOI:10.1038/s41467-020-15060-y


CLASP promotes microtubule bundling in metaphase spindle independently of Ase1/PRC1 in fission yeast
Hirohisa Ebina, Liang Ji and Masamitsu Sato
Biology Open (2019) 8, bio045716 DOI:10.1242/bio.045716


Kinetochore-mediated outward force promotes spindle pole separation in fission yeast
Yutaka Shirasugi and Masamitsu Sato
Molecular Biology of the Cell (2019) 30(22):2802-2813 DOI:10.1091/mbc.E19-07-0366


Module-based systematic construction of plasmids for episomal gene expression in fission yeast
Keita Kiriya, Hayato Tsuyuzaki, Masamitsu Sato
Gene (2017) 637:14-24 DOI:10.1016/j.gene.2017.09.030


Reconstructing regulatory pathways by systematically mapping protein localization interdependency networks
James Dodgson, Anatole Chessel, Federico Vaggi, Marco Giordan, Miki Yamamoto, Kunio Arai, Marisa Madrid, Marco Geymonat, Juan Francisco Abenza, Jose Cansado, Masamitsu Sato, Attila Csikasz-Nagy, Rafael E Carazo-Salas
bioRxiv (2017) 116749 DOI:10.1101/116749


Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore
Takashi Akera, Yuhei Goto, Masamitsu Sato, Masayuki Yamamoto and Yoshinori Watanabe
Nature Cell Biology (2015) 17(9):1124-1133.　DOI:10.1038/ncb3219


Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe
*Yasutaka Kakui, Tomonari Sunaga, Kunio Arai, James Dodgson, Liang Ji, Attila Attila Csikász-Nagy, Rafael Carazo-Salas and *Masamitsu Sato
Open Biology (2015) 5(6):150054. DOI:10.1098/rsob.150054


Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast
Bouhlel IB, Ohta M, Mayeux A, Bordes N, Dingli F, Boulanger J, Velve Casquillas G, Loew D, Tran PT, Masamitsu Sato and *Anne Paoletti
Journal of Cell Science (2015) 128(8):1481-1493. DOI:10.1242/jcs.159657


The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A
Hayato Hirai, Kunio Arai, Ryo Kariyazono, Masayuki Yamamoto and *Masamitsu Sato
PLoS ONE (2014) 9(11):e111905. DOI:10.1371/journal.pone.0111905


Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis
Yuki Aoi, Shigehiro A. Kawashima, Viesturs Simanis, Masayuki Yamamoto, *Masamitsu Sato
Open Biology (2014) 4(7):140063 DOI:10.1098/rsob.140063


CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly
Naoyuki Okada, Takashi Toda, Masayuki Yamamoto and *Masamitsu Sato
Molecular Biology of the Cell (2014) 25(13):1969-1982. DOI:10.1091/mbc.E13-11-0679


Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast
Yuki Aoi, Masamitsu Sato, Takashi Sutani, Katsuhiko Shirahige, Tarun M. Kapoor and *Shigehiro A. Kawashima
Cell Cycle (2014) 13(8):1327-1334. DOI:10.4161/cc.28294


The RNA-binding protein Spo5 promotes meiosis II by regulating cyclin Cdc13 in fission yeast
Mayumi Arata, Masamitsu Sato, Akira Yamashita and *Masayuki Yamamoto
Genes to Cells (2014) 19(3):225-238. DOI:10.1111/gtc.12133


A network approach to mixing delegates at meetings
Federico Vaggi, Tommaso Schiavinotto, Jonathan Ld Lawson, Anatole Chessel, James Dodgson, Marco Geymonat, Masamitsu Sato, Rafael Edgardo Carazo Salas and *Attila Csikász-Nagy
eLife  (2014) 3:e02273. DOI:10.7554/eLife.02273


Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast
Naoyuki Togashi, Akira Yamashita, Masamitsu Sato and *Masayuki Yamamoto
BMC Microbiology (2014) 14:188. DOI:10.1186/1471-2180-14-188


Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
Ngang Heok Tang, Naoyuki Okada, Chii Shyang Fong, Kunio Arai, Masamitsu Sato and *Takashi Toda
FEBS Letters (2014) 588(17):2814-21 DOI:10.1016/j.febslet.2014.06.027


Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation
Yasutaka Kakui, *Masamitsu Sato, Naoyuki Okada, Takashi Toda and Masayuki Yamamoto
Nature Cell Biology (2013) 15(7):786-796. DOI:10.1038/ncb2782


Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control
James Dodgson, Anatole Chessel, Miki Yamamoto, Federico Vaggi, Susan Cox, Edward Rosten, David Albrecht, Marco Geymonat, Attila Csikasz-Nagy, Masamitsu Sato and *Rafael E. Carazo-Salas
Nature Communications (2013) 4:1834 DOI:10.1038/ncomms2813


Cuf2 boosts the transcription of APC/C activator Fzr1 to terminate the meiotic division cycle
Yuki Aoi, Kunio Arai, Masaya Miyamoto, Yuji Katsuta, Akira Yamashita, *Masamitsu Sato and *Masayuki Yamamoto
EMBO Reports (2013) 14(6):553-560. DOI:10.1038/embor.2013.52


Dynamics of SIN asymmetry establishment
Archana Bajpai, Anna Feoktistova, Jun-Song Chen, Dannel McCollum, Masamitsu Sato, Rafael E. Carazo-Salas, Kathleen L. Gould and *Attila Csikász-Nagy
PLoS Computational Biology (2013) 9(7):e1003147. DOI:10.1371/journal.pcbi.1003147


Polo kinase reconstitutes the spindle pole body during fission yeast meiosis
Midori Ohta, *Masamitsu Sato and *Masayuki Yamamoto
Molecular Biology of the Cell (2012) 23(10):1799-1811. DOI:10.1091/mbc.E11-11-0951


Interpolar microtubules are dispensable in fission yeast meiosis II
Takashi Akera, *Masamitsu Sato and *Masayuki Yamamoto
Nature Communications (2012) 3:695 DOI:10.1038/ncomms1725


SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates
Shin-ya Okamoto, *Masamitsu Sato, Takashi Toda and Masayuki Yamamoto
PLoS ONE (2012) 7(1):e0030622, DOI:10.1371/journal.pone.0030622


A novel fission yeast mei4 mutant that allows efficient synchronization of telomere dispersal and the first meiotic division
Yasutaka Kakui, Masamitsu Sato, Kayoko Tanaka and Masayuki Yamamoto.
Yeast (2011) 28(6):467-79 DOI:10.1002/yea.1851


Nuclear compartmentalization is abolished during fission yeast meiosis
Kunio Arai, Masamitsu Sato, Kayoko Tanaka and Masayuki Yamamoto.
Current Biology (2010) 20(21): 1913-8 DOI:10.1016/j.cub.2010.09.004


Fission yeast Pcp1 links polo kinase-mediated mitotic entry to γ-tubulin-dependent spindle formation
Chii Shyang Fong, Masamitsu Sato and Takashi Toda.
EMBO Journal (2010) 29:120-30 DOI:10.1038/emboj.2009.331


Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast
*Masamitsu Sato, Naoyuki Okada, Yasutaka Kakui, Masayuki Yamamoto, Minoru Yoshida and *Takashi Toda.
EMBO reports (2009) 10:1161-7 DOI:10.1038/embor.2009.158


Alp7/TACC is a crucial target in Ran-GTPase-dependent spindle formation in fission yeast.
*Masamitsu Sato and *Takashi Toda.
Nature (2007) 447(7142):334-7 DOI:10.1038/nature05773


γ-tubulin complex-mediated anchoring of spindle microtubules to spindle pole bodies requires Msd1 in fission yeast
Mika Toya, Masamitsu Sato, Uta Haselmann, Kazuhide Asakawa, Damian Brunner, Claude Antony and Takashi Toda.
Nature Cell Biology (2007) 9(6):646-53 DOI: 10.1038/ncb1593


Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment
Kazuhide Asakawa, Mika Toya, Masamitsu Sato, Muneyoshi Kanai, Kazunori Kume, Tetsuya Goshima, Miguel Angel Garcia, Dai Hirata and Takashi Toda.
EMBO reports (2005) 6:1194-1200 DOI:10.1038/sj.embor.7400540


New drug-resistant cassettes for gene disruption and epitope tagging in Schizosaccharomyces pombe
Masamitsu Sato, Susheela Dhut and Takashi Toda.
Yeast (2005) 22:583-591 DOI:10.1002/yea.1233


The roles of fission yeast Ase1 in mitotic cell division, meiotic nuclear oscillation and cytokinesis checkpoint signaling
Akira Yamashita#, Masamitsu Sato#, Akiko Fujita, Masayuki Yamamoto and Takashi Toda. (# These authors contributed equally)
Molecular Biology of the Cell (2005) 16:1378-1395 DOI:10.1091/mbc.e04-10-0859


Interdependency of fission yeast Alp14/TOG and coiled-coil protein Alp7 in microtubule localization and bipolar spindle formation
Masamitsu Sato, Leah Vardy, Miguel Angel Garcia, Nirada Koonrugsa and Takashi Toda.
Molecular Biology of the Cell (2004) 15:1609-22 DOI:10.1091/mbc.e03-11-0837


Deletion of Mia1/Alp7 activates Mad2-dependent spindle assembly checkpoint in fission yeast
Masamitsu Sato, Nirada Koonrugsa, Leah Vardy, Sylvie Tournier, Jonathan B. Millar and Takashi Toda.
Nature Cell Biology (2003) 5:764-766 DOI:10.1038/ncb0903-764


14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2p
Masamitsu Sato, Yoshinori Watanabe, Yuji Akiyoshi, and Masayuki Yamamoto.
Current Biology (2002) 12:141-145 DOI:10.1016/s0960-9822(01)00654-6


Phosphorylation of Mei2 and Ste11 by Pat1 kinase inhibits sexual differentiation via the ubiquitin proteolysis and 14-3-3 protein in fission yeast
Kenji Kitamura, Satoshi Katayama, Susheela Dhut, Masamitsu Sato, Yoshinori Watanabe, Masayuki Yamamoto and Takashi Toda.
Developmental Cell (2001) 1:389-399 DOI:10.1016/s1534-5807(01)00037-5


The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling
Masamitsu Sato, Satoko Shinozaki-Yabana, Akira Yamashita, Yoshinori Watanabe, and Masayuki Yamamoto.
FEBS Letters (2001) 499(3):251-255 DOI:10.1016/s0014-5793(01)02567-4




総説 Review Articles

細胞の目覚め機構：栄養認識からクロマチン制御へ
八重奏衣, *佐藤政充
月刊「細胞」 (2025) 57(5):311-313. [LINK]


Wake-up alarm: virtual time-lapse gene expression landscape illuminates mechanisms underlying dormancy breaking of germinating spores
Hayato Tsuyuzaki, Ryosuke Ujiie, Masamitsu Sato
Current Genetics (2021) 67(4):519-534. DOI:10.1007/s00294-021-01177-0


シングルセルRNA-seq解析から明らかになった分裂酵母の目覚め機構
露崎隼，細川正人，竹山春子，佐藤政充
バイオサイエンスとインダストリー(B&I) (2020) 78(6):507-509 [LINK]


Differentiating the roles of microtubule-associated proteins at meiotic kinetochores during chromosome segregation
*Yasutaka Kakui and *Masamitsu Sato
Chromosoma (2016) 125(2):309-20 DOI:10.1007/s00412-015-0541-x


Spatiotemporal regulation of nuclear transport machinery and microtubule organization
Naoyuki Okada and *Masamitsu Sato
Cells (2015) 4(3):406-426. DOI:10.3390/cells4030406


Projecting cell polarity into the next decade
*Attila Csikász-Nagy, Masamitsu Sato and *Rafael E. Carazo Salas
Phil. Trans. Roy. Soc. B: Biological Sciences (2013) 368(1629). DOI:0.1098/rstb.2013.0001


Meiosis and APC/Cyclosome
*Masamitsu Sato
Encyclopedia of Systems Biology (2012) 1219-1223. DOI:10.1007/978-1-4419-9863-7_113


Space shuttling in the cell: Nucleocytoplasmic transport and microtubule organization during the cell cycle
*Masamitsu Sato and *Takashi Toda.
Nucleus (2010) 1(3): 231-6 DOI:10.4161/nucl.1.3.11443


Visualization of fluorescence-tagged proteins in fission yeast and the analysis of mitotic spindle dynamics using GFP-tubulin under the native promoter
*Masamitsu Sato, Mika Toya and *Takashi Toda.
Methods in Molecular Biology - Mitosis (2009) 545:185-203 DOI:10.1007/978-1-60327-993-2_11


分裂酵母のスピンドル微小管形成は酵母特異的か？
*佐藤政充，戸谷美夏，登田隆
蛋白質核酸酵素 (2008) 53(3):197-206 [LINK]


スピンドル形成と染色体分配
佐藤政充，登田隆
実験医学 (2007) 25(5):697-703 [LINK]


分裂酵母に学ぶスピンドル微小管形成のしくみ
戸谷美夏，佐藤政充，登田隆
実験医学 (2007) 25(14):2185-2188 [LINK]


スピンドルと動原体の結合機構
佐藤政充，登田隆
実験医学 (2005) 23(9):1359-1365 [LINK]


Reconstruction of microtubules: entry into interphase.
Masamitsu Sato and Takashi Toda*
Developmental Cell (2004) 6:456-458 DOI:10.1016/S1534-5807(04)00101-7


KinIとTOGによるスピンドルと動原体の動態制御
佐藤政充，登田隆
実験医学 (2004) 22(18):2612-2618 [LINK]


分裂酵母における減数分裂の開始と進行の分子機構
佐藤政充，山本正幸
医学のあゆみ (2001) 199(13):856-860 [LINK]